The BBC One drama, starring Alan Davies as the eponymous mystery-solver and Sarah Alexander as his wife, Polly, returns for three episodes, beginning with The Letters of Septimus Noone.
The Mystery of the Yellow Room, based on a 19th-century story by Gaston Leroux, is currently thrilling London theatre audiences with its enticing blend of music, romance and sizzling Gothic melodrama.
But events take a sinister turn one night when the show's glamorous singing sensation Juno Pirelli is found horribly stabbed inside a locked dressing room, from which no assailant could possibly have escaped.
No weapon or any other evidence of an intruder can be found, nor any rational explanation for the victim's wounds.
As the actress's life hangs in the balance, her producer and colleagues remain baffled. And attention once again turns to the lateral-thinking Jonathan Creek for a solution to the whole grisly puzzle.
But can Creek - now a happily-married man - even be persuaded to embark upon the investigation? As he and his wife, Polly, struggle to come to terms with a sudden personal tragedy, a series of dark and disturbing family secrets is about to emerge that will throw the couple's whole world into turmoil . . .
The episode will co-star Ali Bastian as Juno Pirelli, Paula Wilcox as Hazel Prosser, Simon Thomas as Christophe Holtz, and Raquel Cassidy as Sharon.